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ATCC mycology strains are shipped either frozen on dry ice, in plastic or glass cryopreservation vials, as lyophilized cultures in glass ampoules or serum vials, or as live cultures in test tubes. Upon receipt of frozen cells, immediately revive cells by thawing and subsequently transferring cells to an appropriate growth medium. If this is not possible, store frozen vials in liquid nitrogen vapor (below -130C). Alternatively, the frozen material can be stored between -70C and -80C for short periods (1 to 5 days); however, viability will decline at temperatures above -130C. Freeze-dried cultures can be stored safely at 4C or colder. Upon receipt, rehydrate or dilute freeze-dried cultures using sterile distilled water or the appropriate medium and incubate under the conditions specified on the product sheet. Live cultures should be immediately transferred to tubes or plates containing the recommended medium and incubated under the appropriate growth conditions. Under no circumstances should live cultures be stored at refrigerated or standard freezer temperatures as this may result in the death of the culture.
The standard procedure for cryopreservation is to freeze material slowly until they reach a temperature below -70C in medium that contains a cryoprotectant. Vials are then transferred to a liquid-nitrogen freezer to maintain cultures at temperatures below -130C.
The standard service plan offers a plethora of features such as password protection and safeguards for several hard drives and partitions. The software is available in 6 languages and allows you to freeze and thaw hard drives selectively. Additional tools are offered with the enterprise and cloud services.
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